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Analysis of larval oyster grazing by flow cytometry ArchiMer
Chretiennot-dinet, Marie-josèphe; Vaulot, Daniel; Galois, Robert; Spano, Anna-maria; Robert, Rene.
The ingestion of 8 algal species by oyster larvae (Crassostrea gigas Thunberg, 1793) was measured by flow cytometry (FCM). In a preliminary experiment, cell size (estimated by light scatter) and chlorophyll fluorescence of 30 algal species were evaluated to select those species which could be mixed together and still be easily discriminated by FCM. Grazing experiments were carried out over 48 h with 6 and 15-day old larvae fed on 3 algal mixtures, each containing 3 different algal species. The concentration of each species was estimated at 0, 6, 12, 24 and 48 h by FCM. Grazing pressure on a given algal species was dependent upon the age of the larvae, the time of the day and the composition of the mixture. Grazing rates of older larvae were about twice...
Tipo: Text Palavras-chave: Oyster larvae; Crassostrea gigas; Grazing; Algae; Selective feeding; Flow cytometry.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00090/20164/17817.pdf
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Measuring selectivity of feeding by estuarine copepods using image analysis combined with microscopic and Coulter counting OMA
Tackx, M.L.M.; Zhu, L.; De Coster, W.; Billones, R.G.; Daro, M.H..
Although estuarine zooplankters are generally believed to be detritivorous, high clearance rates by the estuarine copepods <i>Eurytemora affinis</i> and <i>Acartia tonsa</i> on natural estuarine microplankton have been reported in the literature. In order to enable detection of possible selectivity for these microplankton organisms over detritus, a method that measures clearance rates on total particulate matter is proposed. Image analysis is used to measure copepod gut contents, and combined with Coulter counter measurements of total particulate matter concentration, allows one to calculate clearance rates on the latter. The first results indicate that, in the Westerschelde estuary (SW Netherlands), both <i>E....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Estuarine organisms; Feeding behaviour; Image processing; Selective feeding; Acartia tonsa; Copepoda [copepods]; Eurytemora affinis.
Ano: 1995 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2632
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Phytoplankton composition and selective feeding of the pearl oyster Pinctada margaritifera in the Takapoto lagoon (Tuamotu Archipelago, French Polynesia): in situ study using optical microscopy and HPLC pigment analysis ArchiMer
Loret, P; Pastoureaud, Annie; Bacher, Cedric; Delesalle, B.
The in situ diet of the pearl oyster Pinctada margarifera was determined in the lagoon of Takapoto Atoll by comparing the phytoplankton composition of water and bivalve gut contents using 2 different methods, optical microscopy and HPLC pigment analysis. In order to evaluate the available food resources for pearl oysters in the water column, a new method for estimating the pigment/chlorophyll a (chl a) ratio (based on an inverse analysis) was developed which allowed us to determine the contribution of the main phytoplanktonic groups in terms of chi a. In the water, picocyanobacteria and nanoflagellates predominated, the latter being mainly chlorophytes and prymnesiophytes. Comparisons between the results obtained by the 2 methods of investigation indicated...
Tipo: Text Palavras-chave: Pearl oyster Pinctada margaritifera; Selective feeding; Natural diet; Phytoplankton pigments; Coral reefs; French Polynesia.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00000/10992/7606.pdf
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